Small-angle neutron scattering (SANS) has been carried out to examine the block-copolymer-induced depletion interaction of charged silica nanoparticles in aqueous solution. The measurements have been performed on fixed concentrations (1 and 10 wt. %  ) of anionic Ludox silica nanoparticles having sizes of 8 and 16 nm in the presence of 0.1M  NaCl and varying concentration of polyethylene oxide–polypropylene oxide–polyethylene oxide P85 [(EO) 26   (PO) 39   (EO) 26   ] block copolymer. The presence of the block copolymer induces an attractive depletion interaction between charge-stabilized nanoparticles. The effective interaction of silica nanoparticles is modeled by a combination of two Yukawa potentials accounting for attractive depletion and repulsive electrostatic forces. The depletion interaction is found to be a long-range attraction whose magnitude and range increase with block-copolymer concentration. The depletion interaction is further enhanced by tuning the self-assembly of the block copolymer through the variation of temperature. The increase of the depletion interaction ultimately leads to clustering of nanoparticles and is confirmed by the presence of a Bragg peak in the SANS data. The positioning of the Bragg peak suggests simple-cubic-type packing of particles within the clusters. The scattering from the clusters in the low-Q  region is governed by the Porod scattering, indicating that clusters are quite large (order of microns). The depletion interaction is also found to be strongly dependent on the size of the nanoparticles.